7. , . . : , , , , / . . , . . . : , 2009. . 4-7, 11-12.
8. Montoya, J. G. Toxoplasma gondii / J. G. Montoya, J. S. Remington // Principles and Practice of Infectious Diseases / Ed. G. L. Man-dell, J. E. Bennett, R. Dolin. Philadelphia: Churchill Livingstone, 2000. P. 2858-2888.
9. / ( ): . - 10.09.2008. , 2008. - 100 .
10. Report of the technical consultation on clinical staging of HIV/AIDS and HIV/AIDS case definition for surveillance. Copenhagen, WHO Regional Office for Europe, 2005. http://www.euro. who.int/document/E87956.pdf, accessed 19 December 2006).
25.06.2014

616.1.9-055.5

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TRACKING TECHNIQUES OF TRANSPLANTED MESENCHYMAL STEM CELLS
IN THE RECIPIENT S ORGANISM
A. G. Skuratov, A. N. Lyzikov, D. R. Petrenyov
Gomel State Medical University Institute of Radiobiology NAS of Belarus, Gomel
Objective: to characterize and apply modern methods of tracking of mesenchymal stem cells in a recipient after transplantation in experiment.
Material and methods. Mesenchymal stem cells from adipose tissue (isolation, cultivation, labeling with fluorescent PKH67 and Dil dyes); laboratory Wistar rats; experimental model of CCl4-induced chronic hepatitis; introduction of labeled mesenchymal stem cells in the organism of laboratory animals; fluorescence microscopy of organ cryosections; PCR analysis of the copy number of the Sry gene.
Results. The approved tracking techniques of mesenchymal stem cells have showed the informativity of the cell labeling with fluorescent dyes and futher study of their migration in the recipient's organism during the analysis of fluorescence microscopy of the organ cryosections. It detected cell-size centers of bright yellow-green (PKH 67) or red (Dil) fluorescence colocalized with cell nuclei. To assess engraftment of the cells in the long term we applied the technique to identify Sry gene in female recipients after the transplantation of stem cells isolated from males. It showed the survival of mesenchymal stem cells after 45 days after the transplantation.
Conclusion. The study of the tracking of the transplanted mesenchymal stem cells in the recipient's organism in the early period (1-2 weeks) with the help of the techniques of their "in vivo" labeling with lipophilic fluorescent dyes becomes informative. It is possible to evaluate the engraftment of the cells in the remote post-transplantation period by identifying the Sry gene after related transplantation from donors of different sexes.
Key words: mesenchymal stem cells, fluorescent dyes PKH67 and Dil, fluorescence microscopy, Sry gene, PCR analysis.

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1. , . . : / . . , . . // . . 2010. . 16, 4. . 576-604.
2. , . . : / . . // . 2010. . 52, 11. . 918-920.
3. A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone / H. Zhu [et. al.] // Nat Protoc. 2010. Vol. 5(3). P. 550-600.
4. Barry, F. P. Mesenchymal stem cells: clinical applications and biological characterization / F. P. Barry, J. M. Murphy // Int J Biochem Cell Biol. 2004. Vol. 36(4). P. 568-584.
5. Dan, Y. Y. Liver stem cells: a scientific and clinical perspective / Y. Y. Dan, G. C. Yeoh // J. Gastroenterol. Hepatol. 2008. Vol. 23. P. 687-698.
6. Locating the stem cell niche and tracing hepatocyte lineages in human liver / T. G. Fellous [et al.] // Hepatology. 2009. Vol. 49. P. 1655-1663.
7. Fluorescent cell labeling for in vivo and in vitro cell tracking / P. K. Horan [et. al.] // Methods in Cell Biology. 1990. Vol. 33. P. 469.
8. Gokhale, P. J. A prospective on stem cell research / P. J. Gok-hale, P. W. Andrews // Semin Reprod Med. 2006. Vol. 24(5). P. 289-297.
9. Human adipose tissue is a source of multipotent stem cells / P. A. Zuk [et. al.] // Mol Biol Cell. 2002. Vol. 13(12). P. 4279-4295.
10. Meirelles, Lda S. Methodology, biology and clinical applications of mesenchymal stem cells / Lda. S. Meirelles, N. B. Nardi // Front Biosci. 2009. 14. P. 4281-4298.
11. Meirelles, Lda S. In search of the in vivo identity of mesenchymal stem cells / Lda. S. Meirelles, A. I. Caplan, N. B. Nardi // Stem Cells. 2008. 26(9). P. 2287-2299.
12. Concise review: Therapeutic potential of mesenchymal stem cells for the treatment of acute liver failure and cirrhosis / V. Volarevic [et al.] // Stem Cells. 2014. Aug 22. doi. 10. P. 1002; 1818.
06.11.2014
616.133.3:615.462:616-091

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DYNAMICS OF MORPHOLOGICAL CHANGES IN PLASTIC MATERIALS UNDER THE INFLUENCE OF ARTERIAL BLOOD FLOW IN THE CAROTID BASIN
A. A. Pechionkin, A. A. Lyzikov, S. L. Achinovich, L. A. Martemyanova
Gomel State Medical University Gomel Regional Clinical Oncologic Center
Objective: to study morphological changes in the application of various plastic materials for inclusion of the carotid basin into arterial blood flow.